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Image Search Results
Journal: Redox Biology
Article Title: Identification of proteins and cellular pathways targeted by 2-nitroimidazole hypoxic cytotoxins
doi: 10.1016/j.redox.2021.101905
Figure Lengend Snippet: Effects of N 3 -AZA on GAPDH and GSTP1 protein levels, GAPDH localization and their enzymatic activity. (A) Lysates prepared from N 3 -AZA (or 0.02% DMSO) treated normoxic and hypoxic FaDu cells were processed for western blotting. N 3 -AZA treatment did not alter GAPDH and GSTP1 protein levels regardless of O 2 conditions. Representative immunoblots and quantitation [mean ± S.E.M.] from three independent experiments are displayed. (B) Cells treated with N 3 -AZA (or 0.02% DMSO) under normoxia and hypoxia were processed for immunocytochemistry to monitor GAPDH localization; no change in cellular localization of GAPDH was observed in response to N 3 -AZA treatment. The micrographs are representative of at least three independent experiments; scale bar = 20 μm. (C and D) FaDu cells treated with N 3 -AZA (or 0.02% DMSO) under normoxia and hypoxia were processed for GAPDH activity assay (C) or GST activity assay (D); the enzymatic activities of GAPDH and GST were significantly reduced only in N 3 -AZA treated hypoxic cells. Data represent mean ± S.E.M. from three independent experiments.
Article Snippet: Hypoxic incubations were carried out in a humidified chamber under controlled
Techniques: Activity Assay, Western Blot, Quantitation Assay, Immunocytochemistry
Journal: Redox Biology
Article Title: Identification of proteins and cellular pathways targeted by 2-nitroimidazole hypoxic cytotoxins
doi: 10.1016/j.redox.2021.101905
Figure Lengend Snippet: N 3 -AZA click chemistry as a hypoxia marker. (A–D) FaDu cells, treated with different concentration of N 3 -AZA (or 0.02% DMSO vehicle control) were incubated under normoxia or hypoxia (0.1% O 2 or <0.1% O 2 ), and click chemistry was performed on paraformaldehyde fixed cells using a fluorescently tagged alkyne. N 3 -AZA click staining was present only in drug treated hypoxic cells. Intensity of N 3 -AZA click staining increased with drug concentration and decreased with O 2 levels. (E) N 3 -AZA click staining is concentrated in nucleoli. Micrographs displayed are representative of at least three independent experiments; scale bar = 20 μm.
Article Snippet: Hypoxic incubations were carried out in a humidified chamber under controlled
Techniques: Marker, Concentration Assay, Incubation, Staining